Medical Studies


Latest News

Cornell University

Breast Cancer
Colon Cancer
Fat Loss
Hair Growth
Heart Disease
Lung Cancer
Prostate Cancer
Skin Cancer
  • Apple polyphenol extract was tested against the cholera toxin. Researchers concluded that apple polyphenols can be used to treat cholera patients.

Inhibition by apple polyphenols of ADP-ribosyltransferase activity of cholera toxin and toxin-induced fluid accumulation in mice, Microbiol Immunol. 2002


Microbiol Immunol. 2002;46(4):249-55.

Inhibition by apple polyphenols of ADP-ribosyltransferase activity of cholera toxin and toxin-induced fluid accumulation in mice.

Saito T, Miyake M, Toba M, Okamatsu H, Shimizu S, Noda M.

Department of Molecular Infectiology, Graduate
School of Medicine, Chiba University, Japan.

The effects of crude polyphenol extracted from immature apples on the enzymatic and biological activities of a cholera toxin (CT) were investigated. When the apple polyphenol extract (APE) was examined for properties to inhibit CT-catalyzed ADP-ribosylation of agmatine, it was found that APE inhibited it in a dose-dependent manner. The concentration of APE to inhibit 50% of the enzymatic activity of CT (15 microg/ml) was approximately 8.7 microg/ml. The APE also diminished CT-induced fluid accumulation in two diarrhea models for in vivo mice. In the ligated ileum loops, 25 microg of APE significantly inhibited fluid accumulation induced by 500 ng of CT. In a sealed mouse model, even when APE was administered orally 10 min after a toxin injection, fluid accumulation was significantly inhibited at a comparable dosage. Lineweaver-Burk analysis demonstrated that APE had negative allosteric effects on CT-catalyzed NAD: agmatine ADP-ribosyltransferase. We fractionated the APE into four fractions using LH-20 Sephadex resin. One of the fractions, FAP (fraction from apple polyphenol) 1, which contains non-catechin polyphenols, did not significantly inhibit the CT-catalyzed ADP-ribosylation of agmatine. FAP2, which contains compounds with monomeric, dimeric, and trimeric catechins, inhibited the ADP-ribosylation only partially, but significantly. FAP3 and FAP4, which consist of highly polymerized catechin compounds, strongly inhibited the ADP-ribosylation, indicating that the polymerized structure of catechin is responsible for the inhibitory effect that resides in APE. The results suggest that polymerized catechin compounds in APE inhibit the biological and enzymatic activities of CT and can be used in a precautionary and therapeutic manner in the treatment of cholera patients.

PMID: 12061627 [PubMed - indexed for MEDLINE]

(more research)
Can I just eat apples? That's not what they're using in the labs. Click here
What concentrated products are available? Check the Suppliers page here


Sign up FREE to get the latest news on apple polyphenols:

Your First Name:

Your email:

Be the first to know about new studies, reports and products.